Friday, May 19, 2017

BCM 513-I List of Practicals

List of Practicals (M.Sc. Biotechnology, I-Semester)

  1. Preparation of normal, molar, Parts per million (PPM), and percent solutions.
  2. Preparation of different buffer solutions and measure their pH.
  3. Estimation of given protein concentration by Bradford, Lowry (Folin) protein assay and by Biuret test.
  4. Determination of the isoelectric point of casein (of milk).
  5. Titration of amino acids and find the physical constants.
  6. Qualitative detection of glucose in urine (the Trommer reaction).
  7. Quantitative determination of glucose in blood by a glucose-oxidase method.
  8. Determination of total cholesterol concentration in serum.
  9. Determination of urea concentration in serum and urine.
  10. Determination of saponification value of the fat.
  11. Qualitative tests for carbohydrates(Iodine test, Benedict’s test, Barfoed’s  test, Seliwanoff’s test, Bial’s test)
  12. Separation of amino acids by chromatographic techniques and detection by nin-hydrin test.
  13. Separation of lipids by TLC, and detection.

BCM 513-I Biochemistry Model Question

Model Questions for Biochemistry (M.Sc. Biotechnology, I-Semester)

Long Answer Question: (7.5 × 1 = 7.5) (any one)
1) Explain the biological functions of proteins with examples.
2) Explain the essential amino acids.
3) Explain the Ramachadran plot.

Short Answer Question: (2.5 × 3 = 7.5) (any three)
1) Describe how peptide bond is formed.
2) Draw the structures of non-polar and non-charged aliphatic amino acids.
3) Mention the the enzymes and co-factors involved in hydroxylation of proline and lysine in collagen synthesis.
4) Differentiate between fibrous and globular proteins.
4) Describe the structure of collagen.

Very Short Answer Question: (1 × 5 = 5) (any five)
1) Define denaturation of proteins.
2) Which bonds are flexible for rotation in a dipeptide?
3) Mention the replaced amino acids that leads to sickle cell anaemia.
4) Define the quaternary structure of proteins.
5) What are the major amino acids in collagen?
6) Describe the supersecondary structure of protein.
7) Describe in brief about the structure of haemoglobin.
8) What are chaperones.