BCM 513-I List of Practicals

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List of Practicals (M.Sc. Biotechnology, I-Semester)

1. Preparation of normal, molar, Parts per million (PPM), and percent solutions.
2. Preparation of different buffer solutions and measure their pH.
3. Estimation of given protein concentration by Bradford, Lowry (Folin) protein assay and by Biuret test.
4. Determination of the isoelectric point of casein (of milk).
5. Titration of amino acids and find the physical constants.
6. Qualitative detection of glucose in urine (the Trommer reaction).
7. Quantitative determination of glucose in blood by a glucose-oxidase method.
8. Determination of total cholesterol concentration in serum.
9. Determination of urea concentration in serum and urine.
10. Determination of saponification value of the fat.
11. Qualitative tests for carbohydrates(Iodine test, Benedict’s test, Barfoed’s  test, Seliwanoff’s test, Bial’s test)
12. Separation of amino acids by chromatographic techniques and detection by nin-hydrin test.
13. Separation of lipids by TLC, and detection.