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publication description Urinary tract infection (UTI) is one of the frequently diagnosed infectious diseases which is caused mainly by Escherichia coli. E. coli confers resistance against the two major classes of antibiotics due to the production of extended spectrum β-lactamase enzymes (ESBL), biofilm, etc. Biofilm produced by uropathogenic E. coli (UPEC) protects from host immune system and prevent entry of antimicrobial compounds. The main objective of this cross-sectional study was to determine the correlation of biofilm production and antibiotic resistance as well as to characterize the pgaA and pgaC genes responsible for biofilm formation among uropathogenic ESBL producing E. coli.
Glucose 6 phosphate dehydrogenase deficiency and hemoglobinopathy in South Western Region Nepal: a boon or burden
https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-019-4762-6
Citation based on Google scholar
https://scholar.google.com/citations?hl=en&user=94Y7FOoAAAAJ
| All | Since 2021 | |
|---|---|---|
| Citations | 10,879 | 10,169 |
| h-index | 27 | 24 |
| i10-index | 48 | 42 |
| Title | Journal Name | Year |
| Global burden of 288 causes of death and life expectancy decomposition in 204 countries and territories, 1990–2021 | The Lancet | 2024 |
| Global, regional, and national burden of disorders affecting the nervous system, 1990–2021 | The Lancet Neurology | 2024 |
| Burden of disease scenarios for 204 countries and territories, 2022–2050 | The Lancet | 2024 |
| Global age-sex-specific mortality, life expectancy, and population estimates in 204 countries and territories, 1950–2021 | The Lancet | 2024 |
| Global, regional, and national prevalence and mortality burden of sickle cell disease, 2000–2021 | The Lancet Haematology | 2023 |
| Potential roles of medicinal plants for the treatment of viral diseases focusing on COVID-19: A review | Phytotherapy Research | 2021 |
| Global burden of 292 causes of death in 204 countries and territories, 1990–2023 | The Lancet | 2025 |
| High prevalence of multidrug resistance in bacterial uropathogens from Kathmandu, Nepal | BMC Research Notes | 2012 |
| Evaluation of antibacterial activity of some traditionally used medicinal plants against human pathogenic bacteria | BioMed Research International | 2015 |
| Global, regional, and national age-sex-specific burden of diarrhoeal diseases, 1990–2021 | The Lancet Infectious Diseases | 2025 |
| Global, regional, and national burden of HIV/AIDS, 1990–2021, and forecasts to 2050 | The Lancet HIV | 2024 |
| Evaluation of the alpha-amylase inhibitory activity of Nepalese medicinal plants used in the treatment of diabetes mellitus | Clinical Phytoscience | 2020 |
| Global, regional, and national trends in routine childhood vaccination coverage from 1980 to 2023 with forecasts to 2030 | The Lancet | 2025 |
| Cyclopeptide alkaloids of Ziziphus oxyphylla Edgw as novel inhibitors of alpha-glucosidase enzyme and protein glycation | Phytochemistry Letters | 2011 |
| Extended spectrum beta-lactamase producing uropathogenic Escherichia coli and the correlation of biofilm with antibiotics resistance in Nepal | Annals of Clinical Microbiology and Antimicrobials | 2019 |
| Biologically active C-alkylated flavonoids from Dodonaea viscosa | Archives of Pharmacal Research | 2012 |
| Xanthones inhibitors of alpha-glucosidase and glycation from Garcinia nobilis | Phytochemistry Letters | 2012 |
| Molecular Identification and Antimicrobial Potential of Streptomyces Species from Nepalese Soil | International Journal of Microbiology | 2020 |
| Synthesis and enzyme inhibitory activities of some new pyrazole-based heterocyclic compounds | Medicinal Chemistry Research | 2012 |
| Phytochemical analysis of medicinal plants of Nepal and their antibacterial and antibiofilm activities against uropathogenic Escherichia coli | BMC Complementary Medicine and Therapies | 2021 |
| Chemical constituents of Stereospermum acuminatissimum and their urease and alpha-chymotrypsin inhibitions | Fitoterapia | 2012 |
| Triazole based Schiff bases and their Oxovanadium (IV) complexes: Synthesis, characterization, antibacterial assay, and computational assessments | Heliyon | 2023 |
| Facile Plant Extract Mediated Eco-Benevolent Synthesis and Recent Applications of CaO-NPs: A State-of-the-art Review | Journal of Chemical Reviews | 2020 |
| Biotransformation of dehydroepiandrosterone with Macrophomina phaseolina and beta-glucuronidase inhibitory activity of transformed products | Journal of Enzyme Inhibition and Medicinal Chemistry | 2012 |
| Antibiotics Prescription, Dispensing Practices and Antibiotic Resistance Pattern in Common Pathogens in Nepal: A Narrative Review | Microbiology Insights | 2023 |
| Bioassay-guided isolation of urease and alpha-chymotrypsin inhibitory constituents from the stems of Lawsonia alba Lam. (Henna) | Fitoterapia | 2013 |
| Synthesis, Structure-Activity Relationships Studies of Benzoxazinone Derivatives as alpha-Chymotrypsin Inhibitors | Bioorganic Chemistry | 2017 |
| Total Biomass Carbon Sequestration Ability under the Changing Climatic Condition by Paulownia tomentosa Steud | International Journal of Applied Sciences and Biotechnology | 2018 |
| Novel inhibitors of urease from Corydalis govaniana Wall. | Phytochemistry Letters | 2013 |
| Urease inhibitory constituents from Daphne retusa | Journal of Asian Natural Products Research | 2014 |
| Anticancer and alpha-chymotrypsin inhibiting diterpenes and triterpenes from Salvia leriifolia | Phytochemistry Letters | 2013 |
| Molecular Docking and ADMET Prediction of Compounds from Piper longum L. Detected by GC-MS Analysis in Diabetes Management | Moroccan Journal of Chemistry | 2024 |
| Evaluation of Phytochemical, Antioxidant and Antibacterial Activities of Selected Medicinal Plants | Nepal Journal of Biotechnology | 2021 |
| Challenges and Opportunity of in vitro Propagation of Paulownia tomentosa Steud for Commercial Production in Nepal | International Journal of Applied Sciences and Biotechnology | 2016 |
| Ethno-Medicinal Uses of Piper betel: A Review | Advanced Journal of Chemistry Section B | 2021 |
| Synthesis and Characterization of High Molecular Weight Chitosan, and Antioxidant Activity of Its Chitosan Oligosaccharide Encapsulation | Journal of Nepal Chemical Society | 2021 |
| Oxovanadium (IV) Complexes with Triazole Based Schiff Base Ligands: Synthesis, Characterization and Antibacterial Study | Journal of Nepal Chemical Society | 2021 |
| Synthesis and in vitro alpha-chymotrypsin inhibitory activity of 6-chlorobenzimidazole derivatives | Bioorganic & Medicinal Chemistry | 2016 |
| Clinical and microbiological observational study on AmpC beta-lactamase-producing Enterobacteriaceae in a hospital of Nepal | Brazilian Journal of Infectious Diseases | 2013 |
| Glucose 6 phosphate dehydrogenase deficiency and hemoglobinopathy in South Western Region Nepal: a boon or burden | BMC Research Notes | 2019 |
| Geometrical and thermodynamic stability of govaniadine scaffold adducts with dopamine receptor D1 | Results in Chemistry | 2024 |
| Chemical Constituents of Cichorium intybus and their Inhibitory Effects against Urease and alpha-Chymotrypsin Enzymes | Natural Product Communications | 2011 |
| Selected phytochemicals as potent acetylcholinesterase inhibitors: An in silico prediction | Journal of the Serbian Chemical Society | 2025 |
| In Vitro and In Silico Studies for the Identification of Potent Metabolites of Some High-Altitude Medicinal Plants from Nepal Inhibiting SARS-CoV-2 Spike Protein | Molecules | 2022 |
| Broad spectrum anticancer activity of pistagremic acid | Chemotherapy | 2013 |
| Phytochemical Analysis and Anti-Microbial Activity of Desmostachya bipinnata: A review | Journal of Medicinal and Chemical Sciences | 2021 |
| Global, regional, and national burden of chronic respiratory diseases and impact of the COVID-19 pandemic, 1990–2023 | Nature Medicine | 2026 |
| Salicylaldehyde salicyloyl hydrazone and its copper (II) complex: synthesis, characterization, DFT, optical band gap, antibacterial activity, and molecular docking analysis | Journal of Coordination Chemistry | 2024 |
| Biotransformation of Daidzein, Genistein, and Naringenin by Streptomyces Species Isolated from High-Altitude Soil of Nepal | International Journal of Microbiology | 2021 |
| Isolation and Characterization of Soil Myxobacteria from Nepal | Journal of Institute of Science and Technology | 2019 |
| Two new halocuprates complexes synthesis and characterization | Medicinal Chemistry Research | 2012 |
| Diorganotin (IV) derivative of 2-thiophene acetic acid: characterizations and biological activities | Medicinal Chemistry Research | 2012 |
| Cytotoxic Activity of Alkaloids Isolated from Stephania glandulifera Miers | Journal of Biologically Active Products from Nature | 2023 |
| Biochemical Analysis and Human Aldose Reductase Inhibition Activity of Selected Medicinal Plants of Nepal | Journal of Chemistry | 2023 |
| Phytochemicals of Swertia chirayita Roxb. ex Fleming against malarial dihydroorotate dehydrogenase: an in silico study | Discover Molecules | 2024 |
| Smartphone-operated affordable PCR thermal cycler for the detection of antimicrobial resistant bacterial genes | PLOS Global Public Health | 2023 |
| A Computational Approach of Anti-diabetic Potential Evaluation of Flower and Seed of Nyctanthes arbor tristis Linn | Turkish Computational and Theoretical Chemistry | 2025 |
| Bioactive Molecules Against Malarial Dihydroorotate Dehydrogenase: An in silico Approach | Moroccan Journal of Chemistry | 2024 |
| COVID-19 mortality and its associated factors in Nepal: A cross-sectional study | IJID Regions | 2023 |
| Synthesis, Physicochemical Studies and Biological Applications of Oxovanadium(IV) Complexes With Schiff Bases | International Journal of Advanced Research | 2022 |
| Exploring Artocarpus heterophyllus phytochemicals as novel mineralocorticoid receptor inhibitors | Journal of King Saud University - Science | 2025 |
| AI-assisted discovery of potent FGFR1 inhibitors via virtual screening and in silico analysis | PLOS ONE | 2025 |
| Computational approach for the evaluation of sesquiterpene lactone as a modulator of cannabinoid receptor type 2 | Molecular Diversity | 2025 |
| Inhibitory effects of phytochemicals of spices on human monoamine oxidase B through molecular docking, molecular dynamics simulation, and DFT study | Discover Chemistry | 2025 |
| alpha-Glucosidase Inhibitory Activity of Compounds from the Essential Oil of Leucas lavandulifolia Sm. | Asian Journal of Chemical Sciences | 2024 |
| Spatial and Energetic Stability Assessment of the Adducts of Phytocompounds of Piper longum L. with alpha-amylase | Biointerface Research in Applied Chemistry | 2024 |
| Discovery and Study of the Binding Epitopes of Novel Urease Inhibitors by STD-NMR Spectroscopy and Biochemical Analyses | Letters in Drug Design & Discovery | 2013 |
| Global burden of amphetamine, cannabis, cocaine and opioid use in 204 countries, 1990-2023 | Nature Medicine | 2026 |
| Unveiling the therapeutic potential of artopetelin flavonoids through computational approaches as PPARdelta agonists | Journal of Molecular Graphics and Modelling | 2025 |
| Corrigendum to: Valencene derived from essential oils of Psidium guajava L. as multi-target neurodegenerative inhibitor | Australian Journal of Chemistry | 2025 |
| Evaluating the inhibitory efficacy of Oxalis phytocompounds on monoamine oxidase B | PLOS ONE | 2025 |
| Geometrical and thermodynamical stability of the adducts of the phytochemicals of Swertia chirayita with Plasmodium falciparum protein | Structural Chemistry | 2025 |
| Chemical Analysis and Biological Studies of Leaf Extracts of Smallanthus sonchifolius with In-silico Assessment of GC-MS Identified Compounds | South Asian Research Journal of Natural Products | 2024 |
| Tyrosinase Inhibition Activities of Depsides Isolated from Ramalina Species | Journal of Biologically Active Products from Nature | 2023 |
| Knowledge and DdeI Based Confirmation of Sickle Cell Anemia Among the Tharu Community | Journal of Nepal Health Research Council | 2022 |
| Design and synthesis of potent and selective beta-glucuronidase inhibitor by virtual and in vitro screening | World Journal of Pharmaceutical Sciences | 2014 |
| Alpha-Chymotrypsin and Urease Inhibitory Activity of Diversolides C, D and E from the Roots of Ferula Diversivittata | Journal of the Chemical Society of Pakistan | 2012 |
| Molecular docking and ADMET analysis of phytochemicals of Kalanchoe pinnata as modulators of mineralocorticoid receptor | BIBECHANA | 2025 |
| Dermatophytosis: A Review on Epidemiology, Pathogenesis, Clinical Features, Diagnosis and Treatment Strategies in Nepal | Health Science Reports | 2024 |
| Exploration of Antioxidant, Antibacterial, and Alpha-glucosidase Inhibition Potential of Cirsium verutum Extracts | Asian Journal of Applied Chemistry Research | 2024 |
| nCoV-19 therapeutics using cucurbitacin I structural derivatives: an in silico approach | Future Journal of Pharmaceutical Sciences | 2024 |
| Exploring the Therapeutic Potential of Kalanchoe Pinnata: A Comprehensive Analysis of Bioactive Compounds | Biology, Medicine, & Natural Product Chemistry | 2024 |
| Epidemiological Analysis of Dengue: A Review | International Journal of Medicine & Biomedical Sciences | 2018 |
| THIOUREA DERIVATIVES AS alpha-CHYMOTRYPSIN INHIBITORS | US Patent 20150080580 | 2015 |
| Evaluation of Antimicrobial Activities of Medicinal Plants Against Some Fungi and Antibiotic Resistant Bacteria (MSc Thesis) | Central Department of Microbiology, TU | 2008 |
| Machine Learning-guided Virtual Screening and Molecular Dynamics Simulations for Identifying Potent FGFR1 Inhibitors | Journal of Pharmaceutical Innovation | 2026 |
| Global, regional, and national burden of meningitis, 1990–2023 (GBD Study 2023) | The Lancet Neurology | 2026 |
| Altitudinal and household breeding patterns of mosquitoes Aedes aegypti, Aedes albopictus and Culex quinquefasciatus in Nepal | PLOS ONE | 2026 |
| Computational Evaluation of Phytochemicals from Oxalis as Modulators of Human Pregnane X Receptor | Jordan Journal of Chemistry | 2025 |
| In Silico Docking, Pharmacokinetic, and Toxicological Profiling of Ziziphus budhensis Leaves Phytochemicals as Aldose Reductase Inhibitors | Amrit Research Journal | 2025 |
| Knowledge, attitude, and practice of Sickle cell disease among patients in Sudurpashchim Province of Nepal | Health Science Reports | 2025 |
| Socio-Demographic Characteristics and Clinical profile in Sickle Cell Disease Patients | Journal of Nepal Health Research Council | 2025 |
| Phytochemical, antioxidant, and enzyme inhibition potential exploration of Nyctanthes arbor-tristis | Ecletica Quimica | 2025 |
| Malaria Prophylactics Through Heat Shock Protein 70x Chaperone Inhibition by Alkaloids of Polyalthia longifolia | ChemistrySelect | 2025 |
| National Summit of Health and Population Scientists in Nepal: Achievements and Lesson Learned Over a Decade | Journal of Nepal Health Research Council | 2025 |
| Assessment of preparedness of COVID-19 vaccination Programme at local government level in Nepal | Nepal Health Research Council (Report) | 2025 |
| Study on hemoglobinopathies and G6PD deficiency in Terai districts of Nepal | Nepal Health Research Council (Report) | 2025 |
| An Evaluation of the Anti-diabetic Potential of Rubia manjith Roxburgh ex Fleming Using In Vitro and In Silico Methods | Unpublished/Preprint | 2025 |
| GC-MS Analysis, Oral Toxicity Evaluation, and Molecular Docking Studies of Ocimum tenuiflorum L. Essential Oil | Amrit Research Journal | 2024 |
| Determination of Physicochemical Parameters and Structural Elucidation of Crab-shell Chitosan | Novel Aspects on Chemistry and Biochemistry Vol. 7 (Book Chapter) | 2023 |
| Correlation of Serum Urea, Creatinine and Urinary Microalbumin in Type 2 Diabetic Patients in Western Nepal | International Journal of Health Sciences and Research | 2023 |
| Vaccines and the role of the immune system: An approach in decreasing COVID-19 mortality | International Journal of Medicine and Biomedical Sciences | 2021 |
| In-Silico Analysis of Natural Products that Modulates Enzymes of Diabetic Target | Preprints | 2020 |
| Pharmacophore-based Virtual Screening and In-vitro activity Evaluation of alpha-Chymotrypsin Inhibitors | International Journal of Advanced Research | 2016 |
| NextGen VOICES | Science | 2013 |
| NextGen Speaks | Science | 2012 |
| Anti-Cancer Activity of Tectona hamiltoniana: An Endemic Plant of Myanmar | Journal of the Chemical Society of Pakistan | 2012 |
Facile Plant Extract Mediated Eco-Benevolent Synthesis and Recent Applications of CaO-NPs: A State-of-the-art Review
Full text: jchemrev.com DOI: 10.33945/SAMI/JCR.2020.3.7
Trupti Pagar, Suresh K Ghotekar, Shreyas Pansambal, Bishnu P. Marasini
Full text: jchemrev.com DOI: 10.33945/SAMI/JCR.2020.3.7
Trupti Pagar, Suresh K Ghotekar, Shreyas Pansambal, Bishnu P. Marasini
Ameliorating the sustainable and eco-accommodating routes for the procurable synthesis of nanoparticles (NPs) is a crucial aspect in the area of green nanotechnology. The known conventional routes for the production of NPs are complicated, noxious, expensive and not safer to human and environment. To overcome these threats, natural precursors such as biopolymers, plant, fungi, and bacteria have been used to fabricate the calcium oxide nanoparticles (CaO-NPs). The shape, size, and applications of the CaO-NPs are prominently affected by the reaction parameters under which they are synthesized. Moreover, the CaO-NPs synthesized by green approach have found potential applications in a wide spectrum of areas including, catalysis, bio-ceramics, additive in refractory, biodiesel production, adsorbent, antimicrobial agent, removal of Cr(VI) and trans esterification of oils. This research study discussed various plants and the different plant parts thathave been used for the synthesis of CaO-NPs. The protocol, characterization techniques, mechanism, and eco-benign synthesis of the CaO-NPs along with various recent applications were also discussed.
Isolation and Characterization of Soil Myxobacteria from Nepal
publication date: Dec 31, 2019; publication description Journal of Institute of Science and Technology, 24(2): 7-16
publication description
Realizing myxobacteria as a potential source of antimicrobial
metabolites, we pursued research to isolate myxobacteria showing
antimicrobial properties. We have successfully isolated three strains
(NR-1, NR-2, NR-3) using the Escherichia coli baiting technique. These
isolates showed typical myxobacterial growth characteristics.
Phylogenetic analysis showed that all the strains (NR-1, NR-2, NR-3)
belong to the family Archangiaceae, suborder Cystobacterineae, and order
Myxococcales. Furthermore, 16S rRNA gene sequence similarity searched
through BLAST revealed that strain NR-1 showed the closest similarity
(91.8 %) to the type strain Vitiosangium cumulatum (NR-156939), NR-2
showed (98.8 %) to the type of Cystobacter badius (NR-043940), and NR-3
showed the closest similarity (83.5 %) to the type of strain Cystobacter
fuscus (KP-306730). All isolates showed better growth in 0.5-1 % NaCl
and pH around 7.0, whereas no growth was observed at pH 9.0 and below
5.0. All strains showed better growth at 32° C and hydrolyzed starch,
whereas casein was efficiently hydrolyzed by NR-1 and NR-2. Besides,
preliminary antimicrobial tests from crude extracts showed activities
against Gram-positive, Gram-negative bacteria, and fungi. Our findings
suggest that the arcane soil habitats of Nepal harbor myxobacteria with
the capability to produce diverse antimicrobial activities that may be
explored to overcome the rapidly rising global concern about antibiotic
resistance.
Extended spectrum β-lactamase producing uropathogenic Escherichia coli and the correlation of biofilm with antibiotics resistance in Nepal
publication date Dec 17, 2019 publication description BioMed Central Ltdpublication description Urinary tract infection (UTI) is one of the frequently diagnosed infectious diseases which is caused mainly by Escherichia coli. E. coli confers resistance against the two major classes of antibiotics due to the production of extended spectrum β-lactamase enzymes (ESBL), biofilm, etc. Biofilm produced by uropathogenic E. coli (UPEC) protects from host immune system and prevent entry of antimicrobial compounds. The main objective of this cross-sectional study was to determine the correlation of biofilm production and antibiotic resistance as well as to characterize the pgaA and pgaC genes responsible for biofilm formation among uropathogenic ESBL producing E. coli.
Glucose 6 phosphate dehydrogenase deficiency and hemoglobinopathy in South Western Region Nepal: a boon or burden
https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-019-4762-6
The study was carried out to optimize the
phenotypic method to characterize the sickle cell trait (SCT), sickle
cell anemia (SCA), and β-thalassemia (β-TT) suspected sample from
tharu community of South Western province-5, Nepal. SCT and SCA were
further evaluated by genotypic method employing amplification
refractory mutation system (ARMS PCR). Moreover, Glucose 6 phosphate
dehydrogenase (G6PD) was estimated in those hemoglobinopathy to
observe its prevalence. The accurate and reliable method can play an
important role in reduction of morbidity and mortality
rate.
Results
The 100 suspected cases were subjected to phenotypic method adopting cellulose acetate electrophoresis and genotypic method using ARMS PCR which portraits (5%) SCA positive test showing HBS/HBS, (38%) SCT positive trait HBA/HBS and (36%) cases normal HBA/HBA. β-TT (21%) cases were confirmed by electropherogram. G6PD deficiency was observed in (40%) of SCA, (18.4%) of SCT, (4.8%) of β-TT and (2.8%) in normal cases. Increased G6PD were developed only in SCT (5.3%) and β-TT (4.8%). The study highlighted sickle cell disorder (SCD) and β-TT as the most common hemoglobinopathy coexisting with G6PD deficiency. Though hemoglobinopathy sometime could be protective in malaria but G6PD deficiency can cause massive hemolysis which may exacerbate the condition.
Challenges and Opportunity of in vitro Propagation of Paulownia tomentosa Steud for Commercial Production in Nepal
publication date 2016 publication description International Journal of Applied Sciences and Biotechnology
Evaluation of Antibacterial Activity of Some Traditionally Used Medicinal Plants against Human Pathogenic Bacteria
publication date Jan 16, 2015 publication description BioMed Research International
publication description The worldwide increase of multidrug resistance in both community- and health-care associated bacterial infections has impaired the current antimicrobial therapy, warranting the search for other alternatives. We aimed to find the in vitro antibacterial activity of ethanolic extracts of 16 different traditionally used medicinal plants of Nepal against 13 clinical and 2 reference bacterial species using microbroth dilution method. The evaluated plants species were found to exert a range of in vitro growth inhibitory action against the tested bacterial species, and Cynodon dactylon was found to exhibit moderate inhibitory action against 13 bacterial species including methicillin-resistant Staphylococcus aureus, imipenem-resistant Pseudomonas aeruginosa, multidrug-resistant Salmonella typhi, and S. typhimurium. The minimum inhibitory concentration (MIC) values of tested ethanolic extracts were found from 31 to >25,000 μg/mL. Notably, ethanolic extracts of Cinnamomum camphora, Curculigo orchioides, and Curcuma longa exhibited the highest antibacterial activity against S. pyogenes with a MIC of 49, 49, and 195 μg/mL, respectively; whereas chloroform fraction of Cynodon dactylon exhibited best antibacterial activity against S. aureus with a MIC of 31 μg/mL. Among all, C. dactylon, C. camphora, C. orchioides, and C. longa plant extracts displayed a potential antibacterial activity of MIC < 100 μg/mL.
Early onset Alzheimer’s Disease and its Pathogenesis
publication date 2015 publication description Nepal Med Coll J
publication description Prevalence of Alzheimer’s disease (AD) is significantly high in developed countries where life expectancy is relatively higher and its incidence is expected to increase over years. Etiology of this disease is complex; however, in a subtype called Early Onset Alzheimer’s Disease (EOAD) which manifests at age below 65 years, genes are the sole cause — mutations in APP, PSEN1 and PSEN2 genes. Currently, there is no effective cure for AD. Therefore, a thorough understanding of pathogenesis of AD is crucial for designing drug-development studies. However, our knowledge is only in the growing phase in this field. This paper briefly reviews the pathogenesis of EOAD
Clinical and microbiological observational study on AmpC β-lactamase-producing Enterobacteriaceae in a hospital of Nepal.
publication date Apr 2013 publication description Brazilian Journal of Infectious Diseases
publication description Brazilian Journal of Infectious Diseases, 17 (2), 256-259 doi:10.1016/j.bjid.2012.09.012
Limited information is available regarding AmpC β-lactamase (ABL)-producing Enterobacteriaceae compared to extended-spectrum β-lactamase-producing enterobacteria. Since ABL-producing organisms are often resistant to multiple antimicrobial agents, therapeutic options against these pathogens are limited. Among 230 clinical Enterobacteriaceae isolates, 64 (27.8%) were found to produce ABL in our study. Escherichia coli (83.9%) was a predominant pathogen, followed by Citrobacter freundii (5.2%). A significant proportion of ABL-producing isolates (81.3%) were found to be multidrug resistant against commonly used antibiotics. Univariate analysis showed that prior history of taking antibiotics (odds ratio [OR], 5.278; confidence interval [CI], 2.838–9.817; p < 0.001) and being inpatients (OR, 4.587; CI, 2.132–9.9; p < 0.001) were associated with ABL positivity. Regular antimicrobial resistance surveillance for ABL-producing Enterobacteriaceae is warranted for proper antimicrobial treatment strategy and policy making due to ABL-positive infections.
Nepal's Ancient Technologies Needs Revival to Ameliorate the Economy / NextGenVOICES 5 (6)
publication date Jan 4, 2013 publication description Science
publication description http://www.sciencemag.org/content/339/6115/30/suppl/DC1
DOI: 10.1126/science.339.6115.30
Stop Overuse of Antibiotics / NextGenVOICES 4 (15)
publication date Oct 5, 2012 publication description Science
publication description http://www.sciencemag.org/content/338/6103/40/suppl/DC1
DOI: 10.1126/science.338.6103.40
High prevalence of multidrug resistance in bacterial uropathogens from Kathmandu, Nepal
publication description BMC Research Notes 2012, 5:38 doi:10.1186/1756-0500-5-38
Results
The 100 suspected cases were subjected to phenotypic method adopting cellulose acetate electrophoresis and genotypic method using ARMS PCR which portraits (5%) SCA positive test showing HBS/HBS, (38%) SCT positive trait HBA/HBS and (36%) cases normal HBA/HBA. β-TT (21%) cases were confirmed by electropherogram. G6PD deficiency was observed in (40%) of SCA, (18.4%) of SCT, (4.8%) of β-TT and (2.8%) in normal cases. Increased G6PD were developed only in SCT (5.3%) and β-TT (4.8%). The study highlighted sickle cell disorder (SCD) and β-TT as the most common hemoglobinopathy coexisting with G6PD deficiency. Though hemoglobinopathy sometime could be protective in malaria but G6PD deficiency can cause massive hemolysis which may exacerbate the condition.
Challenges and Opportunity of in vitro Propagation of Paulownia tomentosa Steud for Commercial Production in Nepal
publication date 2016 publication description International Journal of Applied Sciences and Biotechnology
Evaluation of Antibacterial Activity of Some Traditionally Used Medicinal Plants against Human Pathogenic Bacteria
publication date Jan 16, 2015 publication description BioMed Research International
publication description The worldwide increase of multidrug resistance in both community- and health-care associated bacterial infections has impaired the current antimicrobial therapy, warranting the search for other alternatives. We aimed to find the in vitro antibacterial activity of ethanolic extracts of 16 different traditionally used medicinal plants of Nepal against 13 clinical and 2 reference bacterial species using microbroth dilution method. The evaluated plants species were found to exert a range of in vitro growth inhibitory action against the tested bacterial species, and Cynodon dactylon was found to exhibit moderate inhibitory action against 13 bacterial species including methicillin-resistant Staphylococcus aureus, imipenem-resistant Pseudomonas aeruginosa, multidrug-resistant Salmonella typhi, and S. typhimurium. The minimum inhibitory concentration (MIC) values of tested ethanolic extracts were found from 31 to >25,000 μg/mL. Notably, ethanolic extracts of Cinnamomum camphora, Curculigo orchioides, and Curcuma longa exhibited the highest antibacterial activity against S. pyogenes with a MIC of 49, 49, and 195 μg/mL, respectively; whereas chloroform fraction of Cynodon dactylon exhibited best antibacterial activity against S. aureus with a MIC of 31 μg/mL. Among all, C. dactylon, C. camphora, C. orchioides, and C. longa plant extracts displayed a potential antibacterial activity of MIC < 100 μg/mL.
Early onset Alzheimer’s Disease and its Pathogenesis
publication date 2015 publication description Nepal Med Coll J
publication description Prevalence of Alzheimer’s disease (AD) is significantly high in developed countries where life expectancy is relatively higher and its incidence is expected to increase over years. Etiology of this disease is complex; however, in a subtype called Early Onset Alzheimer’s Disease (EOAD) which manifests at age below 65 years, genes are the sole cause — mutations in APP, PSEN1 and PSEN2 genes. Currently, there is no effective cure for AD. Therefore, a thorough understanding of pathogenesis of AD is crucial for designing drug-development studies. However, our knowledge is only in the growing phase in this field. This paper briefly reviews the pathogenesis of EOAD
Clinical and microbiological observational study on AmpC β-lactamase-producing Enterobacteriaceae in a hospital of Nepal.
publication date Apr 2013 publication description Brazilian Journal of Infectious Diseases
publication description Brazilian Journal of Infectious Diseases, 17 (2), 256-259 doi:10.1016/j.bjid.2012.09.012
Limited information is available regarding AmpC β-lactamase (ABL)-producing Enterobacteriaceae compared to extended-spectrum β-lactamase-producing enterobacteria. Since ABL-producing organisms are often resistant to multiple antimicrobial agents, therapeutic options against these pathogens are limited. Among 230 clinical Enterobacteriaceae isolates, 64 (27.8%) were found to produce ABL in our study. Escherichia coli (83.9%) was a predominant pathogen, followed by Citrobacter freundii (5.2%). A significant proportion of ABL-producing isolates (81.3%) were found to be multidrug resistant against commonly used antibiotics. Univariate analysis showed that prior history of taking antibiotics (odds ratio [OR], 5.278; confidence interval [CI], 2.838–9.817; p < 0.001) and being inpatients (OR, 4.587; CI, 2.132–9.9; p < 0.001) were associated with ABL positivity. Regular antimicrobial resistance surveillance for ABL-producing Enterobacteriaceae is warranted for proper antimicrobial treatment strategy and policy making due to ABL-positive infections.
Nepal's Ancient Technologies Needs Revival to Ameliorate the Economy / NextGenVOICES 5 (6)
publication date Jan 4, 2013 publication description Science
publication description http://www.sciencemag.org/content/339/6115/30/suppl/DC1
DOI: 10.1126/science.339.6115.30
Stop Overuse of Antibiotics / NextGenVOICES 4 (15)
publication date Oct 5, 2012 publication description Science
publication description http://www.sciencemag.org/content/338/6103/40/suppl/DC1
DOI: 10.1126/science.338.6103.40
High prevalence of multidrug resistance in bacterial uropathogens from Kathmandu, Nepal
publication description BMC Research Notes 2012, 5:38 doi:10.1186/1756-0500-5-38
https://link.springer.com/article/10.1186/1756-0500-5-38
The impact of Urinary Tract Infection (UTI) is worst in low-resource developing countries due to unaware of the UTIs caused by multidrug-resistant (MDR) pathogens and the possibility of transfer of MDR traits between them. Two hundred and nineteen bacterial isolates were recovered from 710 urine samples at Kathmandu Model hospital during the study period. All samples and isolates were investigated by standard laboratory procedures. Among the significant bacterial growth (30.8%, 219 isolates), 41.1% isolates were MDR. The most prevailing organism, E. coli (81.3%, 178 isolates) was 38.2% MDR, whereas second most common organism, Citrobacter spp. (5%, 11 isolates) was found 72.7% MDR. Extended-spectrum β-lactamase (ESBL) production was detected in 55.2% of a subset of MDR E. coli isolates. Among the 29 MDR E. coli isolates, plasmids of size ranging 2-51 kb were obtained with different 15 profiles. The most common plasmid of size 32 kb was detected in all of the plasmid-harbored E. coli strains. The majority of E. coli isolates investigated for the multidrug resistance transfer were able to transfer plasmid-mediated MDR phenotypes along with ESBL pattern with a frequency ranging from 0.3 × 10-7 to 1.5 × 10-7 to an E. coli HB101 recipient strain by conjugation. Most of the donor and recipient strain showed high levels of minimum inhibitory concentration (MIC) values for commonly-used antibiotics. The high prevalence of multidrug resistance in bacterial uropathogens was observed. Particularly, resistance patterns were alarmingly higher for amoxycillin, co-trimoxazole, flouroquinolones and third-generation cephalosporins, which necessitate the re-evaluation of first and second line therapies for UTI. In addition, conjugational co-transfer of MDR phenotypes with ESBL-positive phenotypes was observed in MDR E. coli.
Total Biomass Carbon Sequestration Ability Under the Changing Climatic Condition by Paulownia tomentosa Steud
publication description International Journal of Applied Sciences and Biotechnology
publication description The concentration of carbon dioxide (CO2) has risen continuously in atmosphere due to human induced activities, and has been considered the predominant cause of global climate change. Paulowina tomentosa Steud. (P. tomentosa), a multipurpose tree is popular around global market for its timber and its potential role in CO2 sequestration. In this study, the total biomass carbon of five years old and newly planted P. tomentosa has been estimated. The results indicated that the average total biomass carbon of five years old plant was found to be 4.52±0.53Kg C Year-1 per tree i.e. 9.04±1.06-ton C ha-1 Year-1 (assuming 2000 plants per hector). Likewise, the average total biomass carbon of newly planted P. tomentosa within 4 months was found to be 6.07±0.38 Kg in remote village area in Nepal. The estimated biomass carbon in one year of newly planted plants was found to be 18.21±1.14 Kg Year-1 i.e. 0.36-ton C ha-1 Year-1. These findings reveled that short rotational trees like P. tomentosa can be implemented in agroforestry system to reduce the green house emission in cities and emphasizes the carbon storage potential of agroforestry. In vitro micro propagation technique could be implemented to produce genetically uniform clone of P. tomentosa and can be applied in agroforestry system for the adaptation and to mitigate global climate change.
The impact of Urinary Tract Infection (UTI) is worst in low-resource developing countries due to unaware of the UTIs caused by multidrug-resistant (MDR) pathogens and the possibility of transfer of MDR traits between them. Two hundred and nineteen bacterial isolates were recovered from 710 urine samples at Kathmandu Model hospital during the study period. All samples and isolates were investigated by standard laboratory procedures. Among the significant bacterial growth (30.8%, 219 isolates), 41.1% isolates were MDR. The most prevailing organism, E. coli (81.3%, 178 isolates) was 38.2% MDR, whereas second most common organism, Citrobacter spp. (5%, 11 isolates) was found 72.7% MDR. Extended-spectrum β-lactamase (ESBL) production was detected in 55.2% of a subset of MDR E. coli isolates. Among the 29 MDR E. coli isolates, plasmids of size ranging 2-51 kb were obtained with different 15 profiles. The most common plasmid of size 32 kb was detected in all of the plasmid-harbored E. coli strains. The majority of E. coli isolates investigated for the multidrug resistance transfer were able to transfer plasmid-mediated MDR phenotypes along with ESBL pattern with a frequency ranging from 0.3 × 10-7 to 1.5 × 10-7 to an E. coli HB101 recipient strain by conjugation. Most of the donor and recipient strain showed high levels of minimum inhibitory concentration (MIC) values for commonly-used antibiotics. The high prevalence of multidrug resistance in bacterial uropathogens was observed. Particularly, resistance patterns were alarmingly higher for amoxycillin, co-trimoxazole, flouroquinolones and third-generation cephalosporins, which necessitate the re-evaluation of first and second line therapies for UTI. In addition, conjugational co-transfer of MDR phenotypes with ESBL-positive phenotypes was observed in MDR E. coli.
Total Biomass Carbon Sequestration Ability Under the Changing Climatic Condition by Paulownia tomentosa Steud
publication description International Journal of Applied Sciences and Biotechnology
publication description The concentration of carbon dioxide (CO2) has risen continuously in atmosphere due to human induced activities, and has been considered the predominant cause of global climate change. Paulowina tomentosa Steud. (P. tomentosa), a multipurpose tree is popular around global market for its timber and its potential role in CO2 sequestration. In this study, the total biomass carbon of five years old and newly planted P. tomentosa has been estimated. The results indicated that the average total biomass carbon of five years old plant was found to be 4.52±0.53Kg C Year-1 per tree i.e. 9.04±1.06-ton C ha-1 Year-1 (assuming 2000 plants per hector). Likewise, the average total biomass carbon of newly planted P. tomentosa within 4 months was found to be 6.07±0.38 Kg in remote village area in Nepal. The estimated biomass carbon in one year of newly planted plants was found to be 18.21±1.14 Kg Year-1 i.e. 0.36-ton C ha-1 Year-1. These findings reveled that short rotational trees like P. tomentosa can be implemented in agroforestry system to reduce the green house emission in cities and emphasizes the carbon storage potential of agroforestry. In vitro micro propagation technique could be implemented to produce genetically uniform clone of P. tomentosa and can be applied in agroforestry system for the adaptation and to mitigate global climate change.
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