Wednesday, July 15, 2015

Structure, properties and classification of amino acids

Some key-words are:
What is the structure ?
What is the role of Proteins?

  1. The function of a protein is intrinsically dependent on its structure.
  2. Since its structure is maintained by combinations of chemical interactions which are individually weak and flexible, the environment in which a protein finds itself can affect its structure.
  3. Heat and pH affect protein structure.
  4. The cell uses factors such as phosphorylation or ligand-binding to affect the structure, and therefore the function, of many proteins
Amino Acid
  1. The first discovered Amino Acid was asparagine, in 1806.
  2. The last, threonine, 1938.
  3. All the amino acids have trivial or common names, in some cases derived from the source from which they were first isolated.
  4. Asparagine was first found in asparagus,
  5. Glutamate in wheat gluten;
  6. Tyrosine was first isolated from cheese (its name is derived from the Greek tyros, “cheese”); and glycine (Greek glykos, “sweet”) was so named because of its sweet taste.
Essential Amino acids: They are not synthesized by the body and must be supplied from the diet. These are; Isoleucine, Leucine, Lysine, Methionine, Phenylanine, Threonine, Tryptophan, Valine, Arginine*, and Histidine*
PVTtim H*A*LL (private team  hall)
*Semi essential amino acid
Rest of the materials are found in pdf file. Please, wait in case of slow internet.

Sunday, July 12, 2015

Model Questions for Biochemistry-III

 Model Questions for Biochemistry (Microbiology) 
1. Explain in brief about lipolysis.
2. Explain the urea cycle.
3. What is the salvage pathway, enlist the steps?
4. Why TCA is said to be amphibolic?
5. What are the ketone bodies? Explain their metabolism.

6. What are enzymes, explain with the classification?
7. Give some examples of the special molecules of the body whose precursor are amino acids.
8. Enlist the enzymes involved in TCA cycle and estimate the number of ATP generation.
9. Describe the stoichiometry for palmitic acid oxidation.
10. What are the differences between fatty acid biosynthesis and breakdown.

11. Derive Michaelis-Menten equation.
12. What are coenzymes and isozymes? Explain in brief with examples.
13. Explain pyruvate dehydrogenase complex.
14. What is the glyoxylate cycle? Show its relationship to the citric acid cycle.
15. Explain the De novo synthesis of Pyrimidine (steps only).