Applied Biotechnology

Tweet

Modern Technique in Biochemistry

1. Describe how affinity chromatography is useful for protein purification. 7
2. Describe the size exclusion gel chromatography in detail.  7
3. Describe the principle, procedure and uses of  paper chromatography.  8
4. What is immunodetection? Describe the fluorescence immunoassay.  2+6
5. Tabulate the difference between RIA and ELISA.    7
6. Describe the principle and instrumentation of PCR.    3+5
7. Explain about the vectors in gene cloning.   7
8. Describe the principle and uses of western blotting.  7
9. Write the Nernst equation.  Write the application of biosensor.  2+5
10. Describe the mechanism of pH electrode.    8
11. What is electrophoretic mobility (μ)? Describe the agarose gel electrophoresis.    2+7
12. How PAGE is prepared, explain.        6
13. What is redox reaction? Write the application of redox couple.    2+5
14. Describe the principle of Clark’s oxygen electrode.    8
15. What is bioinformatics? Mention the scope of bioinformatics in clinical diagnosis.   2+5
16. Enlist the scope of biotechnology.     8

Write a short note    5

• Ion selective electrode
• Hybridization
• DNA sequencing  
• Column chromatography
• Capacity factor in chromatography
• Fluorescence polarization immunoassay
• Electrochemiluminescence