BCM BT513-II Biochemistry Model Question.

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Model Questions for Biochemistry (M.Sc. Biotechnology, I-Semester, set II)

Long Answer Question: (7.5 × 1 = 7.5) (any one)

1. Write the structure and function of polysaccharides in the living organisms.
2. Classify the lipids and describe the structure of saturated and unsaturated fatty acids.
3. Explain the major enzyme-catalyzed reactions with examples.
4. What is PUFA? Describe the structure and functions of steroids.
5. Write the structure and function of mono-saccharides.
6. Define Michaelis-Menten expression for single substrate single enzyme catalyzed reaction. What is the significance of Michaelis constant?

Short Answer Question: (2.5 × 3 = 7.5) (any three)

1. Write a short note on competitive inhibitor.
2. What are the biological roles of nucleic acids?
3. Describe the structure and biological role of glycoprotein.
4. Explain the two major hypotheses for mode of enzyme action.
5. Define enzyme. Describe Six-major types of enzyme-catalysed reaction.
6. Suppose we have a mixture of an enzyme that contains total number of enzyme 0.1M. At this concentration (when all active site are filled), the maximal rate of reaction is 60,000M/S. Calculate the turnover number of that enzyme and time it takes to convert one substrate into a product molecule.
7. Draw the structure of lactose.
8. Draw the structure of sucrose.
9. Write a short note on starch.

Very Short Answer Question: (1 × 5 = 5) (any five)

1. Write the Michaelis-Menten equation.
2. Write the Henderson and Hasselbach equation.
3. What do you mean by Turnover Number of an enzyme?
4. Define cofactor and coenzyme.
5. What is an isoenzyme?
6. What do you mean by steady state assumption?
7. Draw the structure of alpha-D-glucose.
8. Draw the structure of alpha-D-fructose.
9. Draw the structure of dGTP.
10. What is glycogenin?
11. Write a short note on sphingolipid.

 

Molecular and Cell Biology; and Genetics

Long Answer Question: (8)

1. Describe the initiation of the replication process.
2. Describe the structure and type of gene.
3. What is a nucleosome? Describe the formation of a solenoid (condensed fiber) of DNA.
4. What are the functions of RNA? Describe mRNA.
5. Describe transcription briefly (in Prokaryotes).
6. What are the Post-transcriptional Modifications? Describe in brief.
7. Describe the initiation of the translation process in Prokaryotes.
8. What are the Post-translational Modifications? Describe in brief.
9. Describe the type of mutation and results due to base substitution. 
10. Describe in  brief about the chemical mutagens.
11. Describe the lac operon system.
12. How is gene regulation promotor work in prokaryotes? illustrate with diagram.
13. What is DNA cloning? Describe restriction enzymes (type II endonuclease).
14. Illustrate with diagram of pUC19 and pBR322 as cloning vector.
15. What are the applications of genetic engineering in biomedical sciences?
16. Describe phenol-chloroform extraction method of DNA isolation.
17. Describe the southern blot hybridization.
18. Describe the steps in PCR.
19. Highlight some gene editing techniques.
    
    

Short Answer Question: (5)

1. Enlist the enzymes involved in the replication of DNA.
2. Describe the proof-reading process of DNA polymerase.
3. Describe tRNA.
4. Draw the structure of adenine and cytosine. 
5. Write a very short note on the human genome. 
6. List the RNA polymerases and their product in Eukaryotes.
7. Differentiate between RNA polymerase and DNA polymerase.
8. Describe briefly inhibitors of transcription and their mode of action in Prokaryotes.
9. What are the genetic codes and their characters?
10. Describe translocation process during protein synthesis.
11. Shine-Dalgarno sequence. 
12. What is base excision repair.
13. TATA binding protein.
14. Inhibitors of DNases.