My Interest in Isolation and Identification of Escherichia coli from Urine Sample

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The greenish shining colony in M-endo Agar distinguish E. coli from other Coliforms that help to separate E. coli from other bacteria present in stool and urine sample. Also, this method and media can be used to check contamination after antibacterial assay of E. coli. This method saved my time to identify by various sub-culture test.

Isolation and Identification of Salmonella spp. from Clinical/Food Sample

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               The red colonies with black centers and media turn red (phenol red as indicator) in Xylose lysine deoxycholate (XLD) agar media distinguish Salmonella spp. from other coliforms that help to separate from other bacteria present in clinical blood/stool and food sample. Also, this method and media can be used to check contamination after antibacterial assay of Salmonella spp.

               Salmonellae can metabolize thiosulfate and produce hydrogen sulfide, hence black centers seen in colony but Shigellae can't and remains pink colony. Other coliforms are seen as yellow/orange colonies.
                Thus I separated Salmonella spp. very quickly from rest of the organisms.