Finally, we got Publication in International Journal

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After a long waiting, between frustration and enthusiasm, we read enthusiastic and pleasant news about the acceptance of our article in international journal. And surprisingly, it is included in top five "highly accessed" articles for this month (February, 2012).

We had spent almost two years in this research including literature search and writing time. Kathmandu Model Hospital and Central Department of Microbiology had provided lab facility.  Although we had not got any kind of stipend and financial support and even no internet facility in lab or university (it was not free), we did it.  We used to visit Cyber café @ Rs. 20-40 /hour for literature search and related articles. The co-authors, Pankaj Baral and Sanjiv Neupane had spent their pocket money to purchase chemicals such as DNA ladder, restriction enzymes, etc. I spend pocket money for collection of various medicinal plants and some medicines (in kg) that we are using traditionally. Some of our relatives and colleagues used to laugh at us and say it was craziness. This had created a bit disappointment, but we had the enthusiasm for science and research. These days, scientists from most of the developed countries are demanding from Government not to cut the budget for Science and Technology, where as we are struggling for a fraction of amount the budget for Science and Technology.

We would like to thank and express a heartful gratitude to Prof. Dr. Ronald Bauerele, Virginia University School of Medicine, USA, who trained us about the technique of Molecular Biology during six months stay in Nepal. Also, he provided technical and moral support along with science discussion. Thanks to Prof. Dr. Anjana Singh, Central Department of Microbiology, Dr. Bharat Pradhan, Kathmandu Model Hospital and obviously our mentors for guidance and for the arrangement of laboratory.

Anxiety and Anger Soothed by the Pigeons, Symbol of Peace

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I love research work. In childhood, I used to spend several hours on electronics circuit board; forgot hunger-ness, and ultimately scolded by mother. In these days, from  8 AM to 11 PM stay in laboratory (subtracting tea, lunch and dinner time), and sleep immediately after reaching the room. Even in holidays, I have the same routine.

Few days ago, we went in tour. It was a lab missing day for me after several months. All of us had enjoyed the tour. But while returning, my colleague, the newcomer of this institute, noticed the mobile, which was not used for last five hours, was missing from the bag. Oops! The mobile was in bag and the bag was never taken out of the vehicle.  I was among the seniors, who stayed here for a long time, had some responsibility to take care of newcomer, but I, in fact all seniors failed. That created us a pressure and forced to analyze whether the mobile was lost or stolen.

During this 5 hours period we were in three spots. Among 3 spots, the last spot, where vehicle was abandoned for 1 hour while boating to reach middle of the lake, and there were a lot of people in the vicinity of the vehicle. But the driver insisted, “I locked every windows and doors, and there is no sign of opening”. He was the last while leaving the vehicle, and the first while catching up it, after boating. Before and after joining boat, we had spent few minutes on snapping and clicking and were not caring for unlocked vehicle and goods. Probably someone had stolen at that time. The colleague mentioned, "Only mobile is stolen not money and others". This statement made the situation more complex to analyze. We checked the vehicle very carefully, everyone's bag, but no success! Inept! This time, compared to the lab cases that I witnessed, the case is entirely different, no sufficient control group for retrospective study. How can it be interpretable?

Thinking and agitating I couldn't sleep, though I was tired. In the late midnight, I could hear the "coo" of pigeons (probably such sound made in previous days but deep sleep might made unnoticed, I didn't know whether the pigeons were taking shelter in balcony). That 'coo' made irritation to tensioned mind it was like adding ghee in the fire. That increased my anger, I wanted to remove these pigeons from balcony. To appease the anger, I suddenly got up and opened the balcony door, then I found ....Pigeon (Rock Pigeon)......(contd....see part-2)

Survived from Formaldehyde Fuming

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I am preferring the evening/night shift in the crowded cell culture lab to maintain peace. Another lab in day shift. One room is divided in three different compartments each facilitated with laminar hood, inverted microscope, centrifuge, liquid nitrogen tank, and incubators. My cell culture lab is one of them. Last week, as usual, when I entered the lab, pungent like smell and eye irritating environment made me to get out immediately.

I asked a colleague, next to the cell culture lab (thanks at least one was there), whether it was fumigated or not. He replied, “no”, later he said mocking me, “I don't know.” I again returned to the cell culture lab and checked every compartments of the lab and notice board. There should be notice and information for fuming, but nothing could be found as the indicator of fuming except AC was switched off and dustbin was full of waste. “Probably the smell and irritation due to this waste and no air circulation”, I assumed. I had felt such kind of odor on microbiological work, few years ago. Then I decided to work putting mask. Sooner, I felt suffocation and severe headache. It was fuming gas ! ? # Promptly, I rushed to lawn for fresh air breathing.

After 15 minutes, eye irritation reduced but headache persisted then I returned to the room immediately. Thanks I survived. Next day, though appeared in the institute but could not work due to headache. Meanwhile, the same colleague with whom yesterday I asked, bemocked me informing about the fuming. One of the research assistant (or graduate student ?) had fumed with formaldehyde to sterilize one of their compartment at the last time of office hour.

Nepal, Nepali and Blogging; Experience of Nearly a Decade

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       What comes between computer hardware and software? I certainly say, Nepal. Ridiculous, isn’t it? Let me explain. China has been dominating in production of computer parts (hardware) including CPU and India is advance in software design and outsourcing, Nepal is between them (but geographically).
       Could it be possible technically or really towards the development of computer hardware or software or both in New Nepal / e-Nepal? Probably Not! during my life. Success may skew towards software but electricity availability condition …….? Somewhat improvement in IT sectors like telecommunication, Nepal could be an example. But no enough to mention in example, I assume. However, a decade ago, there was trend (fashion) to learn and get trained in basic computer and still it is an essential skill to get any kind of job. Also, it was the matter of self dignity. But it has been limited only in training, not extended to any discovery or industrialization.
       My engrossment was in electronics. Trend to learn computer, and my engrossment / enthusiasm in computer hardware (and still! nevertheless could become a professional), I used to spend several hours in computer related articles, experiments, and in cyber café (Rs. 30-50/hr, later Rs.15-25/hr). http://www.geocities.yahoo.com/bishnu.........(extinct) was the first website (or blog?) that I created. I was at learning stage of html code, jumped from hardware. Initially, it was used to view change in html code after change made using yahoo’s website builder. This site was used for html code practice (observe code, after change in template and vice versa), rather than to publish any article or any advertisement. Later, I used to post some jokes, computer tips (windows, and basic code of html).
       Half of my diary was filled with poems and stories (written in Nepali) and hence, I thought, only thought to post them in the website (geocities). Both, laziness and difficulty in typing and posting in Nepali font (Unicode was unknown to me at that time) made these literature unexposed to public. Later on King Gyanendra’s regime, I found blogspot and wordpress were better to post such kind of literature (in Nepali) and creativity. But the weakness in typing, achieving correct orthography in screen unlike in handwritten manuscript, enervated…oops! Although a revolution made due to Unicode system to post in Nepali, typing time, grammar and punctuation are still making obstacle to post article in Nepali language. I feel odd when reading Nepali article typed by me. I rarely posted these Nepali article (or deleted later) because some word I couldn’t type with correct spelling. I am poor in English, so, I rarely feel odd when reading grammatically mistaken my article.
       Therefore, again, both blogspot and wordpress were used to change template and to design in different ways (wordpress having less option) as in geocities in initial days. Posting and writing couldn’t be continued. Later a decision was made to write article in Nepali for wordpress and in English for blogspot, whatever may be the topic. Article proliferated in blogspot which I hadn’t imagined during opening of its account; just thought Nepali article and literacy will be proliferated as it is easy to create and write; but typing skill made it reverse.
       I salute them (I am not mentioning some important names at this moment, sorry) who not only continuing blog in Nepali but also encouraging other Nepali with correct grammar and spelling, and technical guidance.

Emotional Faith and Peace Philosophy, Incident of Laboratory

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Although I have been hearing lots of stolen cases in this area, I had never witnessed. Even in my lab, there were cases of disappearance of a camera, pen drives, etc....before I joined and/or while I was in hometown. After I joined the lab, the miracle of disappearance of goods had continued, and in last two cases, mobiles were grabbed while all lab members were present, but known only after 2-3 hours. When I got the news, I shocked, how could be possible, all of us were in lab? Immediately, I suggested the lab in-charge to observe CCTV camera (focused to lab's main door from outside). I don't have authority to observe. I don't know whether they observed the whole period of time suspected from the last time phone used to the time of known to stolen. But I guess they did not. At that day, might be all lab fellows were in tension, probably except one, who could grab in front of us.

I witnessed this time but the grabber unknown. What was the motivation and psychology behind there and such mischievous happens (like serial killer in the movie), even in this elite place of well educated scholars? I noted in diary, every situation (I was not in lab in previous cases, witnessed only the last two cases; that's why I could not get sufficient information to analyze). I started the miniature analysis with two hypotheses. I got a lot of information from analysis that took about two months. It was matter of retaliatory and revenge of dissension, which was in climax at that time. Belligerent's intention of bellicose and probably other....... But could not get solid proof that's why it was better not to speak any more in between their emotional belief. I am sure, if lab in charge had investigated from the initial first case, it would be possible to get proof. Probably, their emotional faith and belief or may be question of lab's prestige might became obstacles. Also, I had found the misuse of internet from the log file and tracking software. (I had solid proof in that case). But I never made revealed who was/were culprit(s). Instead, I taught in affable manner which file and how to delete to avoid virus. It is still mysterious why lab in-charge did not investigate.

I (and may be lab in-charge also) may be encouraging and bolstering the culprit(s) by not taking any action. However, we have to see in future. But I want peace, adorable, and family environment in the laboratory, whatever might be some fellows have emotional belief and thinking. Peace !   Peace !!    Peace !!!

Fare

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We headed towards Koteswar from Balkhu, we were five talking about our honesty and innocuity. There was the conductor who had listened our expression. At destination, he asked for fare in greater rate, we discussed a while, but we gave the amount that he asked (greater than ususal).
Then one friend told that he had listened about our innocence, so he asked more fare and we could't resist.

chyura

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When we had gone for a dry picnic, there was "chyura" for us. My friend told me, "I don't like chyura and I know you like most the chyura." I wander and asked how did you know that but I also don't like chyura. He told me, "I always see that lots of packets of chyura when I meet your room." I smiled, wrong interpretation had you ever seen finished that?

A Week Without Contact of Friends

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A Week of few Word Uttered
       Everyday we used to meet at a certain point that would be almost at equal distant for all of us from our room. We used to debate Microbiology, and I was loquacious. If we could not meet, we used to phone one another. But last week became different to me. I did not meet anyone and surprisingly NO phone. I could utter just 2-3 sentences/day, a thrift week.
       I had spent 3 days in IT unit central library where I had spoken only the word, “Namaskar Lal sir.” One day I had planned to meet Khagendra Koirala who had invited me through SMS. But on the road I lured and stuck on cyber café, meanwhile I got an SMS again that he was not in his room, so I continued for five hours in Nayabazaar. Rest of the days I spent in the apartment.
       I was not angry with any of the friends and neither disappointed nor diseased. It was fortuitous. I showed the insanity to study Visual Basic 6.0, VBScript, and web page designing, which I had started to learn many years ago. I wanted a little bit advance study. Fortunately, in the halcyon environment I could study in deep and could generate some files and completed some basic tasks. I could make some amusing web pages as well as some calculating software (that could be useful in our practical class) in Visual Basic. Now I laugh at me why I was spending so many times in computer with such craziness. My profession is not for the computer because my degree in biology.

Not Jokes like joke

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I shook hand with sir
When our department was locked, I shook hand with a sir who was standing in front of channel gate (door), thinking that he was our one batch senior dai and said, "K chha dai? Aaram hununcha?" giving my hand.

Forgot
One of our sir told me to deliver that he would not coming tomorrow to teach us and would come on Tuesday. "Tuesday?" ,I said. "Yes don’t forget to deliver",sir emphasized. "Ok sir". After half an hour when friends met, I said, "sir is not coming tomorrow, he will come on Sunday." (How quickly I forgot).

May be your father
One of our, friend expressed his feeling while entering for tea on a house whose was made up of wood and mud and was squabble. He said, "Oh! like my house, I am feeling I am in my house". Then another friend replied promptly, pointing towards house owner who was cooking tea for us, "He may be your father".

Dog thought
In a picnic spot a dog staring a friend for a long time. The friend was then astonished and told, "Why is it looking at me so long time?" then other friend said, "Perhaps it is thinking about his big brother?"

I realized mistake.

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I got in a microbus from Kalanki for Koteswar toward Sanjeev's room and sat in front sit (not in cabinet). In Balkhu a girl (may be married woman) and a middle aged man entered the microbus and sat in the same sit. The girl was in between me and the man I was in corner toward the window.
I looked outward (I prefer in journey to view seen) and had never bent toward the girl and door after she entered. In Satdobato wari she got off from the microbus. The helper then asked for fare all of us. While giving fare I bent toward the helper (in door side) and I found a fifty rupee note in the sit. Conforming that money was not mine I asked the middle aged man if that belongs to him because he has just taken out money from his pocket to pay fare. At the same time he was giving fare telling he will for Tinkune. After taking money he promptly got off from the microbus in Satdobato pari. Then I realized my mistake.

I lost a thousand rupees

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Perhaps I had put that in virtual pocket of jacket or in side pocket with hanky; I lost a thousand rupees note yesterday. The money was lent by Kasiji to pay my room rent (this is the first time I failed to pay in time although landlord had never asked). It made me very anxious not being able to pay rent in time and losing a big money. I was rebuking myself and no desire to eat at al till late night.

Diving in notion how I can recover that money, lastly I became happy guiding me as successful. Because very rich, merchant traders sometimes loss their money in lakh crore and this is only in thousand showing sign and ignition that I shall also become a rich and genuine.

Then I cooked rice, spicy vegetable & very delicious condiment and enjoyed in eating then slept promptly hoping nice dream.