Monday, February 25, 2019

Applied Biotechnology

Modern Technique in Biochemistry

  1. Describe how affinity chromatography is useful for protein purification. 7
  2. Describe the size exclusion gel chromatography in detail.  7
  3. Describe the principle, procedure and uses of  paper chromatography.  8
  4. What is immunodetection? Describe the fluorescence immunoassay.  2+6
  5. Tabulate the difference between RIA and ELISA.    7
  6. Describe the principle and instrumentation of PCR.    3+5
  7. Explain about the vectors in gene cloning.   7
  8. Describe the principle and uses of western blotting.  7
  9. Write the Nernst equation.  Write the application of biosensor.  2+5
  10. Describe the mechanism of pH electrode.    8
  11. What is electrophoretic mobility (μ)? Describe the agarose gel electrophoresis.    2+7
  12. How PAGE is prepared, explain.        6
  13. What is redox reaction? Write the application of redox couple.    2+5
  14. Describe the principle of Clark’s oxygen electrode.    8
  15. What is bioinformatics? Mention the scope of bioinformatics in clinical diagnosis.   2+5
  16. Enlist the scope of biotechnology.     8

Write a short note    5
  • Ion selective electrode
  • Hybridization
  • DNA sequencing  
  • Column chromatography
  • Capacity factor in chromatography
  • Fluorescence polarization immunoassay
  • Electrochemiluminescence

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