Monday, March 23, 2015

List of Experiments for Biochemistry Laboratory work

                        List of Experiments for Laboratory work
Course Title: Laboratory work I: Biochemistry (Microbiology)
Credit: 1                                                                                                                                  

1. Preparation of normal, molar and percent solutions.
2. Preparation of different buffer solutions and measure their pH.
3. Determination of the isoelectric point of casein (of milk).
4. Titration of amino acids and find the physical constants.
5. Study the effects of temperature and pH on the enzymatic activity of salivary amylase.
6. Estimation of given protein concentration by Bradford, Lowry (Folin) protein assay and by Biuret test.
7. Qualitative detection of glucose in urine (the Trommer reaction).
8. Quantitative determination of glucose in blood by a glucose-oxidase method.
9. The determination of activity of the Krebs cycle dehydrogenases in the liver.
10. Determination of total cholesterol concentration in serum.
11. Determination of urea concentration in serum and urine.
12. Quantitative determination of phospholipids concentration in serum.
13. Determination of saponification value of the fat.
14. Separation of amino acids by chromatographic techniques and detection by nin-hydrin test. 

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